CRBIP-Catalogue

Formulation of medium : 365

MEDIUM 365 - for Desulfovibrio intestinalis

- Di Potassium monohydrogenophosphate 0.5 g (^VWR 26932.290)
- Ammonium chloride 1 g (^Sigma 09718)
- Sodium sulphate 1 g (^Labosi S1660)
- Calcium chloride dihydrate 0.1 g (^Merck 102378)
- Magnesium sulphate heptahydrate 2 g (^Merck 1.05886)
- Sodium lactate 2 g (^Prolabo 27 925.292)
- Yeast extract 1 g (^{BD Difco} 212750)
- Resazurin sodium salt 1 mg (^Sigma 199303)
- Ultra pure water make up to 980 ml (^Millipore MilliQ)
- Solution B - M00807 10 ml
- Solution C - M00852 10 ml

Add all components except solutions B and C Adjust pH to 7.8 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically solutions B and C

Solution B - M00807 10 ml

- Iron (II) sulphate heptahydrate 0.5 g (^Prolabo 24 241.298)
- Ultra pure water make up to 10 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes

Solution C - M00852 10 ml

- Sodium thyoglycolate 0.1 g (^Prolabo 27 938.18)
- Ascorbic acid (vit C) 0.1 g (^Sigma A-5960)
- Ultra pure water make up to 10 ml (^Millipore MilliQ)