CRBIP-Catalogue

Formulation of medium : 381

MEDIUM 381 - for Alkalibacterium

- Soytone bacto 10 g (^{Becton Dickinson} 211677)
- Yeast extract 5 g (^{BD Difco} 212750)
- Glucose 10 g (^Sigma G8270)
- Di Potassium monohydrogenophosphate 1 g (^VWR 26932.290)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 900 ml (^Millipore MilliQ)
- Sodium carbonate solution - M00730 100 ml

Add all products except sodium carbonate solution Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically sodium carbonate solution

Sodium carbonate solution - M00730 100 ml

- Sodium carbonate anhydrous 10 g (^Prolabo 27 771.290)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes