Formulation of medium : 391
MEDIUM 391 - for Filobacillus milosensis
-
Soytone bacto 0.75 g (
Becton Dickinson
211677)
-
Yeast extract 0.75 g (
BD Difco
212750)
-
Glucose 0.75 g (
Sigma
G8270)
-
Sodium chloride 100 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Bacto Agar 18 g (
BD Difco
214010)
-
Ultra pure water make up to 670 ml (
Millipore
MilliQ)
-
Vitamins solution - M00734 10 ml
-
Hunter's Salts solution - M00735 20 ml
-
Tris buffer - M00737 50 ml
-
Synthetic sea solution - M00738 250 ml
Add all products except solutions Adjust pH to 8.5 + 0.2 for solid medium Adjus pH to 7.5 + 0.2 for medium without agar Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically solutions
Vitamins solution - M00734 10 ml
-
Calcium D(+)- pantothenate 5 mg (
BDH
440744Y)
-
Nicotinic acid 5 mg (
Calbiochem
481918)
-
Riboflavin (vit B2) 5 mg (
Sigma
R-4500)
-
Thiamine hydrochloride 5 mg (
Calbiochem
5871)
-
Biotin 2 mg (
Merck
1.24514)
-
Folic acid 2 mg (
Prolabo
20 465.125)
-
Cyanocobalamin (Vit.B12) 0.1 mg (
Calbiochem
6791)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Hunter's Salts solution - M00735 20 ml
-
Nitrilotriacetic acid 10 g (
Sigma
N-9877)
-
Magnesium sulphate heptahydrate 29.7 g (
Merck
1.05886)
-
Molybdic acid tetrahydrate 9.25 mg (
Sigma
M-0878)
-
Calcium chloride dihydrate 3.34 g (
Merck
102378)
-
Iron (II) sulphate heptahydrate 0.09 g (
Prolabo
24 241.298)
-
Metal 44 solution - M00634 M00736 50 ml
-
Ultra pure water make up to 950 ml (
Millipore
MilliQ)
Adjust pH to 6.8 + 0.2 Filter sterilize
Metal 44 solution - M00634 M00736 50 ml
-
Zinc sulphate heptahydrate 1.1 g (
Prolabo
29 158.237)
-
Iron (II) sulphate heptahydrate 0.5 g (
Prolabo
24 241.298)
-
E.D.T.A 0.25 g (
Sigma
E-5134)
-
Manganese II sulphate monohydrate 0.154 g (
Merck
1.05963)
-
Copper II sulphate pentahydrate 0.4 g (
Prolabo
23 174.290)
-
Cobalt II nitrate 0.025 g (
Prolabo
22.913)
-
Di-Sodium tetraborate anhydrous 0.018 g (
Merck
6309)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Tris buffer - M00737 50 ml
-
Trizma hydrochloride 15.76 g (
Sigma
T-3253)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.60 + 0.2 Filter sterilize
Synthetic sea solution - M00738 250 ml
-
Sodium chloride 23.48 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Sodium sulphate 3.92 g (
Labosi
S1660)
-
Magnesium sulphate heptahydrate 4.98 g (
Merck
1.05886)
-
Calcium chloride dihydrate 1.1 g (
Merck
102378)
-
Sodium carbonate hydrogen 0.19 g (
Merck
1.06329)
-
Potassium chloride 0.66 g (
Sigma
P9541)
-
Potassium bromide 6 mg (
Prolabo
26.704)
-
Boric acid 0.026 g (
Prolabo
20 185.297)
-
Strontium chloride hexahydrate 0.024 g (
Sigma
S-0390)
-
Sodium fluoride 3 mg (
Prolabo
27 860.231)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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