CRBIP-Catalogue

Formulation of medium : 397

MEDIUM 397 - for Ruminococcus shinkii

- Cooked meat medium 125 g (^Difco 226730)
- Yeast extract 5 g (^{BD Difco} 212750)
- Di Potassium monohydrogenophosphate 5 g (^VWR 26932.290)
- Resazurin sodium salt 1 mg (^Sigma 199303)
- L-Cystein 0.5 g (^Sigma C7352)
- Glucose 4 g (^Sigma G8270)
- Cellobiose 1 g (^Prolabo 22 558.120)
- Maltose 1 g (^Merck 5910)
- Starch maize 1 g (^Prolabo 21 145.296)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes