Formulation of medium : 399
MEDIUM 399 - for Desulfocella halophila
-
Sodium sulphate 3 g (
Labosi
S1660)
-
Calcium chloride dihydrate 6 g (
Merck
102378)
-
Sodium chloride 40 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Potassium chloride 2 g (
Sigma
P9541)
-
Ammonium chloride 0.3 g (
Sigma
09718)
-
Potassium di-hydrogen phosphate 0.2 g (
Labosi
P1400)
-
Magnesium chloride hexahydrate 8 g (
Merck
1.05833)
-
Strontium chloride hexahydrate 0.1 g (
Sigma
S-0390)
-
Trace elements solution - M00979 1 ml
-
Resazurin sodium salt 0.5 mg (
Sigma
199303)
-
MOPSO(3-[N-Mopholino]-2-hydroxypropanesulfonic acid) 3 g (
Sigma
M-8389)
-
Sodium Pyruvate 3.6 g (
Sigma
P-2256)
-
Yeast extract 1 g (
BD Difco
212750)
-
Sodium carbonate solution - M00442 50 ml
-
Ultra pure water make up to 950 ml (
Millipore
MilliQ)
Add all components except sodium carbonate solution Adjust pH to 7.2 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically sodium carbonate solution
Trace elements solution - M00979 1 ml
-
Hydrochloric acid 25% 10 ml (
Prolabo
20 257.296)
-
Ferrous chloride tetrahydrate 1.5 g (
Merck
1.03861)
-
Zinc chloride 70 mg (
Merck
1.08816)
-
ManganeseII chloride tetrahydrate 100 mg (
Prolabo
25 222.233)
-
Boric acid 6 mg (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 190 mg (
Sigma
C-2644)
-
Copper II chloride dihydrate 2 mg (
Merck
2733)
-
Nickel II chloride hexahydrate 24 mg (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 36 mg (
Merck
6521)
-
E.D.T.A 5.2 g (
Sigma
E-5134)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Sodium carbonate solution - M00442 50 ml
-
Sodium carbonate decahydrate 10 g (
Merck
1.06391.1000)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes.
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