Formulation of medium : 4
MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serum
-
Columbia agar 39 g (
Bio-Rad
279240)
-
Distilled water make up to 1000 ml
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Horse serum 200 ml (
Gibco
26050-088)
Dissolve agar at boiling Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically horse serum
Distilled water make up to 1000 ml
-
Ultra pure water make up to 10000 l (
Millipore
MilliQ)
NULL
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