CRBIP-Catalogue

Formulation of medium : 403

MEDIUM 403 - for Rubrivirax gelatinosus

- Yeast extract 0.3 g (^{BD Difco} 212750)
- Ethanol 0.5 ml (^Merck 1.00983)
- Disodium Succinate hexahydrate / Succinic acid disodium 1 g (^Merck 820151)
- Ammonium acetate 0.5 g (^Merck 1.01116)
- Ferric citrate solution - M00945 5 ml
- Potassium di-hydrogen phosphate 0.5 g (^Labosi P1400)
- Magnesium sulphate heptahydrate 0.4 g (^Merck 1.05886)
- Ammonium chloride 0.4 g (^Sigma 09718)
- Sodium chloride 0.4 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Calcium chloride dihydrate 0.05 g (^Merck 102378)
- Cyanocobalamin solution - M00946 0.4 ml
- Solution SL-6 - M00545 1 ml
- Ultra pure water make up to 1050 ml (^Millipore MilliQ)

Add all components except cyanocobalamin solution Adjust pH to 6.8 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically cyanocobalamin solution

Ferric citrate solution - M00945 5 ml

- Ferric citrate 0.1 g (^Merck 3862)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Cyanocobalamin solution - M00946 0.4 ml

- Cyanocobalamin (Vit.B12) 10 mg (^Calbiochem 6791)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Solution SL-6 - M00545 1 ml

- Zinc sulphate heptahydrate 0.1 g (^Prolabo 29 158.237)
- ManganeseII chloride tetrahydrate 0.03 g (^Prolabo 25 222.233)
- Boric acid 0.3 g (^Prolabo 20 185.297)
- Cobalt II chloride hexahydrate 0.2 g (^Sigma C-2644)
- Copper II chloride dihydrate 0.01 g (^Merck 2733)
- Nickel II chloride hexahydrate 0.02 g (^Prolabo 25 850.290)
- Sodium molybdate dihydrate 0.03 g (^Merck 6521)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Filter sterilize