Formulation of medium : 417
MEDIUM 417 - for Clostridium isotidis
-
Brain heart infusion 17.5 g (
BECTON DICKINSON
237500)
-
Tryptose 10 g (
Becton Dickinson
211713)
-
Glucose 2 g (
Sigma
G8270)
-
Sodium chloride 5 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Di Sodium hydrogen phosphate 2.5 g (
Prolabo
28 026.292)
-
L-Cystein 0.5 g (
Sigma
C7352)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes
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