Formulation of medium : 421
MEDIUM 421 - for Tannerella forsythensis
-
Trypto casein soy agar 40 g (
Bio-Rad
64554)
-
N-Acetylmuranic acid 100 mg (
Sigma
A-3007)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Sheep blood 50 ml (
BioMérieux
55 823)
Add all products except sheep blood Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically sheep blood
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