Formulation of medium : 424
MEDIUM 424 - for Clostridium fimetarium
-
Di Potassium monohydrogenophosphate 0.348 g (
VWR
26932.290)
-
Potassium di-hydrogen phosphate 0.227 g (
Labosi
P1400)
-
Ammonium chloride 0.5 g (
Sigma
09718)
-
Sodium chloride 2.25 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium sulphate heptahydrate 0.5 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.25 g (
Merck
102378)
-
Iron (II) sulphate heptahydrate 2 g (
Prolabo
24 241.298)
-
Solution SL-10 - M00835 1 ml
-
Tungstate sodium selenite solution - M00857 1 ml
-
Vitamins solution - M00861 10 ml
-
Yeast extract 1 g (
BD Difco
212750)
-
Sodium carbonate hydrogen 3 g (
Merck
1.06329)
-
Resazurin sodium salt 0.5 mg (
Sigma
199303)
-
L-Cystein 0.3 g (
Sigma
C7352)
-
Sodium sulphide 0.03 g (
Prolabo
28 164.232)
-
Ultra pure water make up to 990 ml (
Millipore
MilliQ)
Add all components except vitamins solutions Adjust pH to 7 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically vitramins solution
Solution SL-10 - M00835 1 ml
-
Hydrochloric acid 25% 10 ml (
Prolabo
20 257.296)
-
Ferrous chloride tetrahydrate 1.5 g (
Merck
1.03861)
-
Zinc chloride 70 mg (
Merck
1.08816)
-
ManganeseII chloride tetrahydrate 100 mg (
Prolabo
25 222.233)
-
Boric acid 6 mg (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 190 mg (
Sigma
C-2644)
-
Copper II chloride dihydrate 2 mg (
Merck
2733)
-
Nickel II chloride hexahydrate 24 mg (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 36 mg (
Merck
6521)
-
Ultra pure water make up to 990 ml (
Millipore
MilliQ)
Tungstate sodium selenite solution - M00857 1 ml
-
Sodium hydroxyde 0.5 g (
Prolabo
28 252.293)
-
Sodium selenite 3 mg (
Prolabo
28.074)
-
Sodium tungstate 4 mg (
Prolabo
28 190.298)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Vitamins solution - M00861 10 ml
-
Biotin 2 mg (
Merck
1.24514)
-
Folic acid 2 mg (
Prolabo
20 465.125)
-
Pyridoxal hydrochloride (vit B6) 10 mg (
BDH
440865Q)
-
Thiamine hydrochloride 5 mg (
Calbiochem
5871)
-
Riboflavin (vit B2) 5 mg (
Sigma
R-4500)
-
Nicotinic acid 5 mg (
Calbiochem
481918)
-
Calcium D(+)- pantothenate 5 mg (
BDH
440744Y)
-
Cyanocobalamin (Vit.B12) 0.1 mg (
Calbiochem
6791)
-
Para-aminobenzoic acid (vit H) 5 mg (
Sigma
A-9878)
-
Dl-Thioctic Acid 5 mg (
Acros
FW 206.32)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Reduce anaerobically under 5% CO2 + 95% N2 Filter sterilize
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