CRBIP-Catalogue

Formulation of medium : 432

MEDIUM 432 - for Streptomonospora salina

- L-Asparagine 1 g (^Prolabo 21.627)
- Glycerol 10 g (^Prolabo 24 387.292)
- Di Potassium monohydrogenophosphate 1 g (^VWR 26932.290)
- Trace salt solution - M00447 1 ml
- Sodium chloride 150 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes.

Trace salt solution - M00447 1 ml

- Iron (II) sulphate heptahydrate 0.1 g (^Prolabo 24 241.298)
- ManganeseII chloride tetrahydrate 0.1 g (^Prolabo 25 222.233)
- Zinc sulphate heptahydrate 0.1 g (^Prolabo 29 158.237)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Filter sterilize