Formulation of medium : 440
MEDIUM 440 - for Bordetella
-
Bordet gengou agar 30 g (
Becton Dickinson
248200)
-
Glycerol 10 ml (
Prolabo
24 387.292)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Sheep blood 200 ml (
BioMérieux
55 823)
Add all products except sheep blood Adjust pH to 7.4 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically sheep blood
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