CRBIP-Catalogue

Formulation of medium : 460

MEDIUM 460 - for Acholeplasma vituli

- PPLO broth 11 g (^Difco 255420)
- Tryptone 10 g (^{BECTON DICKINSON} 211705)
- Glucose 5 g (^Sigma G8270)
- Ultra pure water make up to 615 ml (^Millipore MilliQ)
- CMRL-1066 (10X) without L-glutamine 50 ml (^Gibco 21540-026)
- Yeast nitrogen base 35 ml (^{BECTON DICKINSON} 239210)
- Yeastolate 100 ml (^{Becton Dickinson} 255772)
- Calf serum 170 ml (^Gibco 10099-141)
- 0.1 % Phenol red - M00387 20 ml

Adjust pH to 7.5 + 0.2 Autoclave at 121°C for 15 minutes.

0.1 % Phenol red - M00387 20 ml

- Sodium hydroxyde 5 ml (^Prolabo 28 252.293)
- Phenol red 500 mg (^Merck 7241)
- Ultra pure water make up to 500 ml (^Millipore MilliQ)

Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.