Formulation of medium : 462
MEDIUM 462 - for Rodophila globiformis
-
Yeast extract 0.25 g (
BD Difco
212750)
-
Mannitol 1.5 g (
Merck
1.05982)
-
Sodium gluconate 0.5 g (
Merck
822058)
-
Potassium di-hydrogen phosphate 0.5 g (
Labosi
P1400)
-
Magnesium sulphate heptahydrate 0.4 g (
Merck
1.05886)
-
Sodium chloride 0.4 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Ammonium chloride 0.4 g (
Sigma
09718)
-
Calcium chloride dihydrate 0.05 g (
Merck
102378)
-
Solution SL-6 - M00545 1 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Ferric citrate 0.1 % solution - M00885 5 ml
-
Biotin 0.002 % solution - M00886 1 ml
-
Para-aminobenzoic acid 0.01 % solution - M00887 1 ml
Adjust pH to 4.9 + 0.2 Autoclave at 121°C for 15 minutes.
Solution SL-6 - M00545 1 ml
-
Zinc sulphate heptahydrate 0.1 g (
Prolabo
29 158.237)
-
ManganeseII chloride tetrahydrate 0.03 g (
Prolabo
25 222.233)
-
Boric acid 0.3 g (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 0.2 g (
Sigma
C-2644)
-
Copper II chloride dihydrate 0.01 g (
Merck
2733)
-
Nickel II chloride hexahydrate 0.02 g (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 0.03 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Ferric citrate 0.1 % solution - M00885 5 ml
-
Ferric citrate 0.1 g (
Merck
3862)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes.
Biotin 0.002 % solution - M00886 1 ml
-
Biotin 0.002 g (
Merck
1.24514)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Para-aminobenzoic acid 0.01 % solution - M00887 1 ml
-
Para-aminobenzoic acid (vit H) 0.01 g (
Sigma
A-9878)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
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