Formulation of medium : 463
MEDIUM 463 - for Pasteurella skyensis
-
Trypto casein soy agar 40 g (
Bio-Rad
64554)
-
Sodium chloride 15 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Ultra pure water make up to 900 ml (
Millipore
MilliQ)
-
Horse blood 100 ml (
BioMérieux
55 833)
Add all products except horse blood Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically horse blood
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