Formulation of medium : 473
MEDIUM 473 - for Tindallia californiensis
-
Sodium chloride 30 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Sodium carbonate decahydrate 2.76 g (
Merck
1.06391.1000)
-
Sodium carbonate hydrogen 24 g (
Merck
1.06329)
-
Potassium chloride 0.2 g (
Sigma
P9541)
-
Di Potassium monohydrogenophosphate 0.2 g (
VWR
26932.290)
-
Magnesium chloride hexahydrate 0.1 g (
Merck
1.05833)
-
Ammonium chloride 1 g (
Sigma
09718)
-
Sodium sulphide 0.4 g (
Prolabo
28 164.232)
-
Resazurin sodium salt 1 mg (
Sigma
199303)
-
Yeast extract 0.5 g (
BD Difco
212750)
-
Soytone bacto 5 g (
Becton Dickinson
211677)
-
Vitamins solution - M00861 2 ml
-
Solution SL-10B - M00917 1 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all products except vitamins solution Adjust pH to 9.5 + 0.2 Prepare medium anaerobically under 5% CO2 + 95% N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically vitamins solution
Vitamins solution - M00861 2 ml
-
Biotin 2 mg (
Merck
1.24514)
-
Folic acid 2 mg (
Prolabo
20 465.125)
-
Pyridoxal hydrochloride (vit B6) 10 mg (
BDH
440865Q)
-
Thiamine hydrochloride 5 mg (
Calbiochem
5871)
-
Riboflavin (vit B2) 5 mg (
Sigma
R-4500)
-
Nicotinic acid 5 mg (
Calbiochem
481918)
-
Calcium D(+)- pantothenate 5 mg (
BDH
440744Y)
-
Cyanocobalamin (Vit.B12) 0.1 mg (
Calbiochem
6791)
-
Para-aminobenzoic acid (vit H) 5 mg (
Sigma
A-9878)
-
Dl-Thioctic Acid 5 mg (
Acros
FW 206.32)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Reduce anaerobically under 5% CO2 + 95% N2 Filter sterilize
Solution SL-10B - M00917 1 ml
-
Hydrochloric acid 25% 7.7 ml (
Prolabo
20 257.296)
-
Iron (II) sulphate heptahydrate 1.5 g (
Prolabo
24 241.298)
-
Zinc chloride 70 mg (
Merck
1.08816)
-
ManganeseII chloride tetrahydrate 100 mg (
Prolabo
25 222.233)
-
Boric acid 300 mg (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 190 mg (
Sigma
C-2644)
-
Copper II chloride dihydrate 2 mg (
Merck
2733)
-
Nickel II chloride hexahydrate 24 mg (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 36 mg (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
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