Formulation of medium : 486
MEDIUM 486 - for Akkermansia muciniphila
-
Solution 1 - M00927 15 ml
-
Solution 2 - M00927 15 ml
-
Solution 3A - M00927 12.5 ml
-
Solution 8 - M00927 1 ml
-
Solution trace 1 - M00926 1 ml
-
Solution trace 2 - M00926 1 ml
-
Rumen fluid 2.5 ml
-
Solution 4 - M00928 50 ml
-
Solution 7 - M00928 1 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes.
Solution 1 - M00927 15 ml
-
Potassium di-hydrogen phosphate 27.2 g (
Labosi
P1400)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution 2 - M00927 15 ml
-
Di Sodium hydrogen phosphate 35.6 g (
Prolabo
28 026.292)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution 3A - M00927 12.5 ml
-
Ammonium chloride 24 g (
Sigma
09718)
-
Sodium chloride 24 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 8 g (
Merck
1.05833)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution 8 - M00927 1 ml
-
Resazurin sodium salt 0.5 g (
Sigma
199303)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution trace 1 - M00926 1 ml
-
Hydrochloric acid 25% 1.8 g (
Prolabo
20 257.296)
-
Boric acid 61.8 mg (
Prolabo
20 185.297)
-
ManganeseII chloride tetrahydrate 61.25 mg (
Prolabo
25 222.233)
-
Ferrous chloride tetrahydrate 943.5 mg (
Merck
1.03861)
-
Cobalt II chloride anhydrous 64.5 mg (
Merck
802540)
-
Nickel II chloride hexahydrate 12.86 mg (
Prolabo
25 850.290)
-
Zinc chloride 67.7 mg (
Merck
1.08816)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution trace 2 - M00926 1 ml
-
Sodium hydroxyde 400 mg (
Prolabo
31 627.290)
-
Sodium selenite 17.3 mg (
Prolabo
28.074)
-
Sodium tungstate 29.4 mg (
Prolabo
28 190.298)
-
Sodium molybdate dihydrate 20.5 mg (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution 4 - M00928 50 ml
-
Sodium carbonate hydrogen 80 g (
Merck
1.06329)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution 7 - M00928 1 ml
-
Sodium sulphide 240.2 g (
Prolabo
28 164.232)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
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