Formulation of medium : 491
MEDIUM 491 - for Caldanaerobacter subterraneus
-
Ammonium chloride 1 g (
Sigma
09718)
-
Di Potassium monohydrogenophosphate 0.3 g (
VWR
26932.290)
-
Potassium di-hydrogen phosphate 0.3 g (
Labosi
P1400)
-
Magnesium chloride hexahydrate 0.2 g (
Merck
1.05833)
-
Potassium chloride 0.1 g (
Sigma
P9541)
-
Calcium chloride dihydrate 0.1 g (
Merck
102378)
-
Yeast extract 2 g (
BD Difco
212750)
-
Sodium chloride 0.5 g (
SIGMA -ALDRICH
31434-1KG-R)
-
L-Cystein 0.5 g (
Sigma
C7352)
-
Resazurin sodium salt 0.001 g (
Sigma
199303)
-
Balch solution - M00838 10 ml
-
Sodium thiosulphate pentahydrate 3.16 g (
Merck
6516)
-
Glucose 3.96 g (
Sigma
G8270)
-
Sodium sulphide 1 g (
Prolabo
28 164.232)
-
Sodium carbonate hydrogen 2 g (
Merck
1.06329)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7 + 0.2 Prepare medium anaerobically under 5% CO2 + 95% N2 gas mixture Autoclave at 121°C for 15 minutes.
Balch solution - M00838 10 ml
-
Nitrilotriacetic acid 1.5 g (
Sigma
N-9877)
-
Magnesium chloride hexahydrate 2.5 g (
Merck
1.05833)
-
Sodium chloride 1 g (
SIGMA -ALDRICH
31434-1KG-R)
-
ManganeseII chloride tetrahydrate 0.6 g (
Prolabo
25 222.233)
-
Ferrous chloride tetrahydrate 100 mg (
Merck
1.03861)
-
Cobalt II chloride hexahydrate 100 mg (
Sigma
C-2644)
-
Calcium chloride dihydrate 100 mg (
Merck
102378)
-
Zinc chloride 10 mg (
Merck
1.08816)
-
Copper II chloride dihydrate 10 mg (
Merck
2733)
-
Aluminium chlorid 10 mg (
Sigma
A-3017)
-
Boric acid 10 mg (
Prolabo
20 185.297)
-
Sodium molybdate dihydrate 10 mg (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 6.5 + 0.2
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