CRBIP-Catalogue

Formulation of medium : 494

MEDIUM 494 - for Trichococcus sp.

- Sodium chloride 5 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Potassium di-hydrogen phosphate 0.3 g (^Labosi P1400)
- Potassium chloride 0.3 g (^Sigma P9541)
- Magnesium sulphate heptahydrate 0.1 g (^Merck 1.05886)
- Calcium sulphate dihydrate 0.013 g (^Prolabo 22 452.237)
- Ammonium chloride 1 g (^Sigma 09718)
- Sodium carbonate hydrogen 0.3 g (^Merck 1.06329)
- Sodium sulphide 0.4 g (^Prolabo 28 164.232)
- Resazurin sodium salt 0.1 mg (^Sigma 199303)
- Glucose 5 g (^Sigma G8270)
- Yeast extract 0.5 g (^{BD Difco} 212750)
- Vitamins solution - M00828 2 ml
- Balch solution - M00838 1 ml
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Add all components except vitamins solution Adjust pH to 7.4 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically vitamins solution.

Vitamins solution - M00828 2 ml

- Biotin 2 mg (^Merck 1.24514)
- Folic acid 2 mg (^Prolabo 20 465.125)
- Pyridoxal hydrochloride (vit B6) 10 mg (^BDH 440865Q)
- Thiamine hydrochloride 5 mg (^Calbiochem 5871)
- Ascorbic acid (vit C) 5 mg (^Sigma A-5960)
- Riboflavin (vit B2) 5 mg (^Sigma R-4500)
- Nicotinic acid 5 mg (^Calbiochem 481918)
- Calcium D(+)- pantothenate 5 mg (^BDH 440744Y)
- Cyanocobalamin (Vit.B12) 0.1 mg (^Calbiochem 6791)
- Para-aminobenzoic acid (vit H) 5 mg (^Sigma A-9878)
- Dl-Thioctic Acid 5 mg (^Acros FW 206.32)
- Choline 5 mg (^Sigma C-7017)
- Myo-inositol 5 mg (^Merck 1.04728)
- Niacinamide (vit B3) 5 mg (^Sigma N-5535)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Balch solution - M00838 1 ml

- Nitrilotriacetic acid 1.5 g (^Sigma N-9877)
- Magnesium chloride hexahydrate 2.5 g (^Merck 1.05833)
- Sodium chloride 1 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- ManganeseII chloride tetrahydrate 0.6 g (^Prolabo 25 222.233)
- Ferrous chloride tetrahydrate 100 mg (^Merck 1.03861)
- Cobalt II chloride hexahydrate 100 mg (^Sigma C-2644)
- Calcium chloride dihydrate 100 mg (^Merck 102378)
- Zinc chloride 10 mg (^Merck 1.08816)
- Copper II chloride dihydrate 10 mg (^Merck 2733)
- Aluminium chlorid 10 mg (^Sigma A-3017)
- Boric acid 10 mg (^Prolabo 20 185.297)
- Sodium molybdate dihydrate 10 mg (^Merck 6521)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 6.5 + 0.2