Formulation of medium : 564
MEDIUM 564 - for Dichelobacter nodosus
-
Beef extract 125 g (
Oxoïd
L29)
-
Trypticase peptone 30 g (
Becton Dickinson
211921)
-
Yeast extract 5 g (
BD Difco
212750)
-
Di Potassium monohydrogenophosphate 5 g (
VWR
26932.290)
-
Bacto Agar 20 g (
BD Difco
214010)
-
L-Cystein 0.5 g (
Sigma
C7352)
-
Bovin hemin solution (0,5g/L) - M00817 10 ml
-
Vitamin K1 solution - M00818 0.2 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all components except Hemin and vitamin K1 solutions Adjust pH to 7 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically Hemin and vitamin K1 solutions.
Bovin hemin solution (0,5g/L) - M00817 10 ml
-
Bovin hemin 0.05 g (
Sigma
H9039)
-
Sodium hydroxyde 30% 500 µl (
Fisher
S/4950/PB15)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize on 0.22µm Keeps for 3 weeks
Vitamin K1 solution - M00818 0.2 ml
-
Vitamin K1 0.15 ml (
Sigma
V-3501)
-
Alcool 95° solution - M0383 30 ml
Alcool 95° solution - M0383 30 ml
-
Alcool ethylique 100° 100 ml (
Magasin général
44654)
-
Distilled water 6.5 ml
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