Formulation of medium : 574
MEDIUM 574 - for Alisonella histaminiformis
-
Di Potassium monohydrogenophosphate 292 mg (
VWR
26932.290)
-
Potassium di-hydrogen phosphate 292 mg (
Labosi
P1400)
-
Ammonium sulphate 480 mg (
Merck
1211)
-
Sodium chloride 480 mg (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium sulphate heptahydrate 100 mg (
Merck
1.05886)
-
Calcium chloride dihydrate 64 mg (
Merck
102378)
-
L-Cystein 500 mg (
Sigma
C7352)
-
Trypticase peptone 1 g (
Becton Dickinson
211921)
-
Yeast extract 4 g (
BD Difco
212750)
-
D-Histidine 7.76 g (
Sigma
H-3751)
-
Sodium carbonate decahydrate 4 g (
Merck
1.06391.1000)
-
Mixed acids - M01042 3.1 ml
-
Resazurin sodium salt 1 mg (
Sigma
199303)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 6 + 0.2 Reduce anaerobically under 5 % CO2, 95 % N2 gas mixture for 4 hours Autoclave at 121°C for 15 minutes
Mixed acids - M01042 3.1 ml
-
Acetic acid 4.25 ml (
Prolabo
20 104.243)
-
Propionique acid 1.5 ml (
Sigma
P-1880)
-
Butyric acid 1 ml (
Sigma
B103500)
-
n - Valeric acid 0.25 ml (
Sigma
V-9759)
-
Isobutyric acid 0.25 ml (
Sigma
I-1754)
-
Methylbutyric acid 0.25 ml (
Sigma
24552-6)
-
Isovaleric acid 0.25 ml (
Acros
156690100)
For a volume : 7.5 ml
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