Formulation of medium : 576
MEDIUM 576 - for Porphyrobacter cryptus
-
Thermus macronutrients 10X solution - M00976 100 ml
-
Thermus micronutrients 100X solution - M00975 10 ml
-
Ferrous chloride - M00977 10 ml
-
Yeast extract 1 g (
BD Difco
212750)
-
Tryptone 1 g (
BECTON DICKINSON
211705)
-
Bacto Agar 20 g (
BD Difco
214010)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 8.2 + 0.2 Autoclave at 121°C for 15 minutes.
Thermus macronutrients 10X solution - M00976 100 ml
-
Nitrilotriacetic acid 1000 mg (
Sigma
N-9877)
-
Calcium sulphate dihydrate 600 mg (
Prolabo
22 452.237)
-
Magnesium sulphate heptahydrate 1000 mg (
Merck
1.05886)
-
Sodium chloride 800 mg (
SIGMA -ALDRICH
31434-1KG-R)
-
Potassium nitrate 1030 mg (
Prolabo
26 869.234)
-
Sodium nitrate 6890 mg (
Prolabo
27.955)
-
Di Sodium hydrogen phosphate 1110 mg (
Prolabo
28 026.292)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes
Thermus micronutrients 100X solution - M00975 10 ml
-
Manganese II sulphate monohydrate 220 mg (
Merck
1.05963)
-
Zinc sulphate heptahydrate 50 mg (
Prolabo
29 158.237)
-
Boric acid 50 mg (
Prolabo
20 185.297)
-
Copper II sulphate pentahydrate 2.5 mg (
Prolabo
23 174.290)
-
Sodium molybdate dihydrate 2.5 mg (
Merck
6521)
-
Cobalt II chloride hexahydrate 4.6 mg (
Sigma
C-2644)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes
Ferrous chloride - M00977 10 ml
-
Ferrous chloride tetrahydrate 46.6 mg (
Merck
1.03861)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
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