Formulation of medium : 578
MEDIUM 578 - for Sporomusa aerovorans
-
Solution A - M00986 907 ml
-
Solution SL-10 - M00835 1 ml
-
Vitamins solution - M00856 1 ml
-
Tungstate sodium selenite solution - M00857 1 ml
-
Yeast extract 1 g (
BD Difco
212750)
-
Casamino acids 1 g (
Becton Dickinson
223050)
-
Solution E - M00987 50 ml
-
DL - dithiothreitol 1M solution - M00990 20 ml
-
Solution F - M00988 20 ml
-
Solution G - M00989 10 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all components except vitamins solution Adjust pH to 7 + 0.2 Prepare medium anaerobically under 5 % CO2 + 95 % N2 gas mixture Autoclave at 121°C for 15 minutes Add aseptically vitamins solution.
Solution A - M00986 907 ml
-
Potassium di-hydrogen phosphate 0.2 g (
Labosi
P1400)
-
Ammonium chloride 0.25 g (
Sigma
09718)
-
Sodium chloride 1 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 0.4 g (
Merck
1.05833)
-
Potassium chloride 0.5 g (
Sigma
P9541)
-
Calcium chloride dihydrate 0.15 g (
Merck
102378)
-
Yeast extract 0.2 g (
BD Difco
212750)
-
Resazurin sodium salt 0.5 g (
Sigma
199303)
-
Ultra pure water make up to 907 ml (
Millipore
MilliQ)
Solution SL-10 - M00835 1 ml
-
Hydrochloric acid 25% 10 ml (
Prolabo
20 257.296)
-
Ferrous chloride tetrahydrate 1.5 g (
Merck
1.03861)
-
Zinc chloride 70 mg (
Merck
1.08816)
-
ManganeseII chloride tetrahydrate 100 mg (
Prolabo
25 222.233)
-
Boric acid 6 mg (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 190 mg (
Sigma
C-2644)
-
Copper II chloride dihydrate 2 mg (
Merck
2733)
-
Nickel II chloride hexahydrate 24 mg (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 36 mg (
Merck
6521)
-
Ultra pure water make up to 990 ml (
Millipore
MilliQ)
Vitamins solution - M00856 1 ml
-
Cyanocobalamin (Vit.B12) 100 mg (
Calbiochem
6791)
-
Para-aminobenzoic acid (vit H) 80 mg (
Sigma
A-9878)
-
Biotin 20 mg (
Merck
1.24514)
-
Nicotinic acid 200 mg (
Calbiochem
481918)
-
Calcium D(+)- pantothenate 100 mg (
BDH
440744Y)
-
Pyridoxal hydrochloride (vit B6) 300 mg (
BDH
440865Q)
-
Thiamine hydrochloride 200 mg (
Calbiochem
5871)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Tungstate sodium selenite solution - M00857 1 ml
-
Sodium hydroxyde 0.5 g (
Prolabo
28 252.293)
-
Sodium selenite 3 mg (
Prolabo
28.074)
-
Sodium tungstate 4 mg (
Prolabo
28 190.298)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution E - M00987 50 ml
-
Sodium carbonate hydrogen 5 g (
Merck
1.06329)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
DL - dithiothreitol 1M solution - M00990 20 ml
-
DL-Dithiothreitol 15.42 g (
Sigma
D-0632)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Solution F - M00988 20 ml
-
Sodium lactate 25 g (
Prolabo
27 925.292)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Solution G - M00989 10 ml
-
Sodium sulphide 0.3 g (
Prolabo
28 164.232)
-
Ultra pure water make up to 10 ml (
Millipore
MilliQ)
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