Formulation of medium : 579
MEDIUM 579 - for Methylocella silvestris
-
Sodium nitrate 0.2 g (
Prolabo
27.955)
-
Magnesium sulphate heptahydrate 0.2 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.06 g (
Merck
102378)
-
E.D.T.A 0.004 g (
Sigma
E-5134)
-
Trace elements solution - M01002 0.5 ml
-
Bacto Agar 15 g (
BD Difco
214010)
-
Solution 3 - M01004 10 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all components except trace elements solution and solution 3 Adjust pH to 5.8 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically above solutions
Trace elements solution - M01002 0.5 ml
-
E.D.T.A 5 g (
Sigma
E-5134)
-
Copper II chloride dihydrate 0.1 g (
Merck
2733)
-
Iron (II) sulphate heptahydrate 2 g (
Prolabo
24 241.298)
-
Zinc sulphate heptahydrate 0.1 g (
Prolabo
29 158.237)
-
Nickel II chloride hexahydrate 0.02 g (
Prolabo
25 850.290)
-
Cobalt II chloride hexahydrate 0.2 g (
Sigma
C-2644)
-
Sodium molybdate dihydrate 0.03 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution 3 - M01004 10 ml
-
Solution 1 - M01003 7.91 ml
-
Solution 2 - M01003 12.09 ml
Adjust pH to 5.8 + 0.2
Solution 1 - M01003 7.91 ml
-
Citric acid 1.92 g (
Sigma
C-1056)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Solution 2 - M01003 12.09 ml
-
Di Sodium hydrogen phosphate 2.84 g (
Prolabo
28 026.292)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Close
Processing
processing in progress, please wait...