Formulation of medium : 584
MEDIUM 584 - for Herminiimonas fonticola
-
Solution A - M01006 50 ml
-
Solution B - M01007 850 ml
-
Solution C - M01008 20 ml
-
Solution D - M01009 30 ml
-
Solution E - M01010 50 ml
-
Vitamins solution - M01011 5 ml
Mix aseptically all solutions
Solution A - M01006 50 ml
-
Potassium di-hydrogen phosphate 2.3 g (
Labosi
P1400)
-
Di Sodium hydrogen phosphate 2.9 g (
Prolabo
28 026.292)
-
Ultra pure water make up to 50 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes
Solution B - M01007 850 ml
-
Ammonium chloride 1 g (
Sigma
09718)
-
Magnesium sulphate heptahydrate 0.5 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.01 g (
Merck
102378)
-
ManganeseII chloride tetrahydrate 5 mg (
Prolabo
25 222.233)
-
Sodium vanate 5 mg (
Prolabo
28 198.238)
-
Solution SL-6 - M00545 50 ml
-
Ultra pure water make up to 850 ml (
Millipore
MilliQ)
-
Bacto Agar 15 g (
BD Difco
214010)
Autoclave at 121°C for 15 minutes
Solution SL-6 - M00545 50 ml
-
Zinc sulphate heptahydrate 0.1 g (
Prolabo
29 158.237)
-
ManganeseII chloride tetrahydrate 0.03 g (
Prolabo
25 222.233)
-
Boric acid 0.3 g (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 0.2 g (
Sigma
C-2644)
-
Copper II chloride dihydrate 0.01 g (
Merck
2733)
-
Nickel II chloride hexahydrate 0.02 g (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 0.03 g (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Solution C - M01008 20 ml
-
Ammonium iron (III) citrate 0.05 g (
Prolabo
24 145.230)
-
Ultra pure water make up to 20 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes
Solution D - M01009 30 ml
-
Yeast extract 1 g (
BD Difco
212750)
-
Sodium acetate 1 g (
Prolabo
27 652.298)
-
Disodium Succinate hexahydrate / Succinic acid disodium 1 g (
Merck
820151)
-
D-L malic acid 1 g (
Prolabo
20 365.233)
-
Ultra pure water make up to 30 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes
Solution E - M01010 50 ml
-
Sodium lactate 1 g (
Prolabo
27 925.292)
-
Sodium Pyruvate 1 g (
Sigma
P-2256)
-
Mannitol 1 g (
Merck
1.05982)
-
Glucose 2 g (
Sigma
G8270)
-
Ultra pure water make up to 50 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Filter sterilize
Vitamins solution - M01011 5 ml
-
Riboflavin (vit B2) 10 mg (
Sigma
R-4500)
-
Thiamine hydrochloride 50 mg (
Calbiochem
5871)
-
Nicotinic acid 50 mg (
Calbiochem
481918)
-
Pyridoxal hydrochloride (vit B6) 50 mg (
BDH
440865Q)
-
Calcium D(+)- pantothenate 50 mg (
BDH
440744Y)
-
Biotin 0.1 mg (
Merck
1.24514)
-
Folic acid 0.2 mg (
Prolabo
20 465.125)
-
Cyanocobalamin (Vit.B12) 1 mg (
Calbiochem
6791)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
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