Formulation of medium : 588
MEDIUM 588 - for Chlorobium
-
Solution A - M00866 4000 ml
-
Solution B - M00867 860 ml
-
Solution C - M00868 5 ml
-
Solution SL-10B - M00917 5 ml
-
Solution E - M00870 100 ml
-
Solution F - M00871 20 ml
Prepare medium anaerobically under 5% CO2 + 95% N2 gas mixture Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 45 minutes Preservation 24 H before use
Solution A - M00866 4000 ml
-
Calcium chloride dihydrate 1.25 g (
Merck
102378)
-
Potassium di-hydrogen phosphate 1.7 g (
Labosi
P1400)
-
Aluminium chlorid 1.7 g (
Sigma
A-3017)
-
Potassium chloride 1.7 g (
Sigma
P9541)
-
Magnesium sulphate heptahydrate 15 g (
Merck
1.05886)
-
Sodium chloride 100 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Ultra pure water make up to 4000 ml (
Millipore
MilliQ)
Autoclave at 121°C for 45 minutes.
Solution B - M00867 860 ml
-
Ultra pure water make up to 860 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes.
Solution C - M00868 5 ml
-
Cyanocobalamin (Vit.B12) 0.002 g (
Calbiochem
6791)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Solution SL-10B - M00917 5 ml
-
Hydrochloric acid 25% 7.7 ml (
Prolabo
20 257.296)
-
Iron (II) sulphate heptahydrate 1.5 g (
Prolabo
24 241.298)
-
Zinc chloride 70 mg (
Merck
1.08816)
-
ManganeseII chloride tetrahydrate 100 mg (
Prolabo
25 222.233)
-
Boric acid 300 mg (
Prolabo
20 185.297)
-
Cobalt II chloride hexahydrate 190 mg (
Sigma
C-2644)
-
Copper II chloride dihydrate 2 mg (
Merck
2733)
-
Nickel II chloride hexahydrate 24 mg (
Prolabo
25 850.290)
-
Sodium molybdate dihydrate 36 mg (
Merck
6521)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Solution E - M00870 100 ml
-
Sodium carbonate decahydrate 7.5 g (
Merck
1.06391.1000)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Solution F - M00871 20 ml
-
Sodium sulphide 10 g (
Prolabo
28 164.232)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes.
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