Formulation of medium : 589
MEDIUM 589 - for Arcobacter holophilus
-
Nutrient agar 1,5% 20 g (
Difco
269100)
-
Sodium chloride 30 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Sheep blood 50 ml (
BioMérieux
55 823)
-
Ultra pure water make up to 950 ml (
Millipore
MilliQ)
Add all components except sheep blood Adjust pH to 7 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically sheep blood
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