CRBIP-Catalogue

Formulation of medium : 592

MEDIUM 592 - for No name sp.

- Yeast extract 1 g (^{BD Difco} 212750)
- Proteose Peptone n°3 1 g (^Difco 211693)
- Casamino acids 1 g (^{Becton Dickinson} 223050)
- Glucose 1 g (^Sigma G8270)
- Sodium Pyruvate 0.05 g (^Sigma P-2256)
- Magnesium sulphate heptahydrate 0.1 g (^Merck 1.05886)
- Di Potassium monohydrogenophosphate 0.6 g (^VWR 26932.290)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Thermus macronutrients 10X solution - M00976 50 ml
- Thermus micronutrients 100X solution - M00975 5 ml
- Tris buffer - M01040 100 ml
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH 8 + 0.2 Autoclave at 121°C for 15 minutes.

Thermus macronutrients 10X solution - M00976 50 ml

- Nitrilotriacetic acid 1000 mg (^Sigma N-9877)
- Calcium sulphate dihydrate 600 mg (^Prolabo 22 452.237)
- Magnesium sulphate heptahydrate 1000 mg (^Merck 1.05886)
- Sodium chloride 800 mg (^{SIGMA -ALDRICH} 31434-1KG-R)
- Potassium nitrate 1030 mg (^Prolabo 26 869.234)
- Sodium nitrate 6890 mg (^Prolabo 27.955)
- Di Sodium hydrogen phosphate 1110 mg (^Prolabo 28 026.292)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes

Thermus micronutrients 100X solution - M00975 5 ml

- Manganese II sulphate monohydrate 220 mg (^Merck 1.05963)
- Zinc sulphate heptahydrate 50 mg (^Prolabo 29 158.237)
- Boric acid 50 mg (^Prolabo 20 185.297)
- Copper II sulphate pentahydrate 2.5 mg (^Prolabo 23 174.290)
- Sodium molybdate dihydrate 2.5 mg (^Merck 6521)
- Cobalt II chloride hexahydrate 4.6 mg (^Sigma C-2644)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes

Tris buffer - M01040 100 ml

- Trizma base 24.2 g (^Sigma T-1503)
- Maleic acid 23.2 g (^Sigma M-9009)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 8 + 0.2 Autoclave at 121°C for 15 minutes