Formulation of medium : 6
MEDIUM 6 - Columbia agar with 10 % horse blood
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Columbia agar 39 g (
Bio-Rad
279240)
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Ultra pure water make up to 1000 mL (
Millipore
MilliQ)
-
Horse blood 100 mL (
BioMérieux
55 833)
Dissolve agar at boiling Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically horse blood
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