Formulation of medium : 602
MEDIUM 602 - for Mycobacterium boletii
-
Middlebrook 7H10 agar 17 g (
BECTON DICKINSON
262710)
-
Glycerol 4.5 ml (
Prolabo
24 387.292)
-
Middelbrook OADC enrichment 100 ml (
Becton Dickinson
211886)
-
Sheep blood 50 ml (
BioMérieux
55 823)
-
Ultra pure water make up to 900 ml (
Millipore
MilliQ)
Add all products except sheep blood and Middlebrook OADC enrichment Adjust pH to 6.6 + 0.2 Autoclave at 121°C for 45 minutes Cool to 50°C Add aseptically sheep blood and Middlebrook OADC enrichment
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