Formulation of medium : 603
MEDIUM 603 - for Helicobacter ganmani
-
Wilkins-Chalgren anaerobe agar 43 g (
sigma
CM619)
-
Sheep blood 100 ml (
BioMérieux
55 823)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
1) Dissolve the Wilkins Chalgren Anaerobe Agar in 500 mL of boiling distilled water 2) Shake using a magnetic stirrer until dissolution is complete 3) Adjust pH 7.1 +/- 0.2 4) Complete to final volume (1 L) 5) Distribute the medium in 500 ml flasks 6) Sterilize for 15 minutes at 121°C 7) Stabilize the bottles at 50°C in a water bath 8) Aseptically add 50 mL of sheep blood per vial of medium 9) Aseptically aliquot 8 mL medium per sterile screw tube 17 10) Place the tubes on a slightly inclined slope.
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