Formulation of medium : 66
MEDIUM 66 - for Propionibacterium propionicus
-
Trypto casein soy broth 30 g (
Bio-Rad
64144)
-
Yeast extract 20 g (
BD Difco
212750)
-
L-Cystein 0.5 g (
Sigma
C7352)
-
Glucose 5 g (
Sigma
G8270)
-
Hemin solution - M00149 25 ml
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 875 ml (
Millipore
MilliQ)
-
Horse blood 125 ml (
BioMérieux
55 833)
Add all products except horse blood and hemin solution Adjust pH to 7.2 + 0.2 Autoclave at 110°C for 30 minutes Cool to 50°C Add aseptically horse blood and hemin solution
Hemin solution - M00149 25 ml
-
Hemin chloride 0.1 g (
Merck
3741)
-
Sodium hydroxyde 30% 0.1 ml (
Fisher
S/4950/PB15)
-
Triethanolamine 4 ml (
VWR
28743.290)
-
Ultra pure water make up to 96 ml (
Millipore
MilliQ)
Filter sterilize
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