Formulation of medium : 67
MEDIUM 67 - for Rarobacter faecitabidus
-
Brain heart infusion 37 g (
BECTON DICKINSON
237500)
-
Hemin solution - M00149 1 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Add all products except hemin solution Adjust pH to 7.4 + 0.2 Autoclave at 110°C for 15 minutes Cool to 50°C Add aseptically hemin solution
Hemin solution - M00149 1 ml
-
Hemin chloride 0.1 g (
Merck
3741)
-
Sodium hydroxyde 30% 0.1 ml (
Fisher
S/4950/PB15)
-
Triethanolamine 4 ml (
VWR
28743.290)
-
Ultra pure water make up to 96 ml (
Millipore
MilliQ)
Filter sterilize
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