Formulation of medium : 672
MEDIUM 672 -for Cystobacter
-
Yeast Baker 5 g
-
Calcium chloride dihydrate 1.36 g (
Merck
102378)
-
Cyanocobalamin (Vit.B12) 0.05 g (
Calbiochem
6791)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 1 l (
Millipore
MilliQ)
Supplier references provided for information only. Final pH : 7,0 0,2 Sterilization Time : 15 min. Temperature : 121°C OPERATING PROCEDURE 1. Mix and dissolve the B12 vitamin in 250 ml of distilled water 2. Sterilize by filtering with Stéricup 3. Mix and dissolve the remaining of the products in 750 ml of distilled water 4. Adjust pH 5. Pack in 1L tube 6. Sterilize 7. After sterilizing, put the tube in a bain-marie at 50°C 8. Once the temperature hit, add aseptically B12 vitamin solution, pour 250 ml in the 1L tube 9. Blend 10. Pour 8 ml of it in sterile 17 ml screw tube 11. Place the tubes slightly sloping CONTROL Make sterility test CONSERVATION CONDITIONS Temperature : keep refrigerated Date of expiry : 1 month
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