Formulation of medium : 678
MEDIUM 678 - for Bacillus okhensis
-
Glucose 10 g (
Sigma
G8270)
-
Potassium di-hydrogen phosphate 10 g (
Labosi
P1400)
-
Soytone bacto 5 g (
Becton Dickinson
211677)
-
Yeast extract 5 g (
BD Difco
212750)
-
Sodium chloride 50 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 9 with 20 % Na2CO3 solution Autoclave at 121°C for 15 minutes
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