Formulation of medium : 682
MEDIUM 682 - for Ornithinibacillus californiensis
-
Nutrient agar 1,5% 31 g (
Difco
269100)
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Sodium chloride 30 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium chloride hexahydrate 5 g (
Merck
1.05833)
pH final : 7,2 ± 0,2 Sterilization Temperature = 121°C Time = 15 min MODE OPERATOIRE - Boil 800 ml of distilled water in a bécher - Add all the products and mix until complete dissolution - Complete with distilled water until the maximum volume - Adjust pH - Divide in 17 ml screw tubes, 8 ml per tube - Sterilize - Place the tubes on a slighty inclined slope 3. CONTROL Make a sterility test CONDITIONS OF CONSERVATION Temperature : + 20°C Date of expiry : one year
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