Formulation of medium : 683
MEDIUM 683 - for Saccharopolyspora cebuensis
-
Starch maize 10 g (
Prolabo
21 145.296)
-
Yeast extract 4 g (
BD Difco
212750)
-
Soytone bacto 2 g (
Becton Dickinson
211677)
-
Bacto Agar 18 g (
BD Difco
214010)
-
Synthetic sea solution - M00738 1000 ml
Adjust pH to 7.0 + 7.2 Autoclave at 121°C for 15 minutes
Synthetic sea solution - M00738 1000 ml
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Sodium chloride 23.48 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Sodium sulphate 3.92 g (
Labosi
S1660)
-
Magnesium sulphate heptahydrate 4.98 g (
Merck
1.05886)
-
Calcium chloride dihydrate 1.1 g (
Merck
102378)
-
Sodium carbonate hydrogen 0.19 g (
Merck
1.06329)
-
Potassium chloride 0.66 g (
Sigma
P9541)
-
Potassium bromide 6 mg (
Prolabo
26.704)
-
Boric acid 0.026 g (
Prolabo
20 185.297)
-
Strontium chloride hexahydrate 0.024 g (
Sigma
S-0390)
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Sodium fluoride 3 mg (
Prolabo
27 860.231)
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Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
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