Formulation of medium : 685
MEDIUM 685 - for Alicyclobacillus melbournensis
-
Tryptone 5 g (
BECTON DICKINSON
211705)
-
D + Glucose 0.5 g (
SIGMA
G 5146)
-
Ammonium sulphate 0.1 g (
Merck
1211)
-
Iron (II) sulphate heptahydrate 70 mg (
Prolabo
24 241.298)
-
Bacto Agar 15 g (
BD Difco
214010)
pH final : 5.5 Sterilization Time : 15 mn Temperature : 121°C OPERATING PROCEDURE 1. Pour 800 ml of distilled water in a bécher 2. Add the products 3. Then complete to the maximum volume 4. Mix until complete dissolution 5. Adjust pH 6. Split in 17 ml screw tubes, so 8ml / tube 7. Then sterilize 8. Place the tubes on a slightly inclined slope CONDITIONS DE CONSERVATION Temperature : + 20 °C
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