Formulation of medium : 69
MEDIUM 69 - for Rhodopseudomonas palustris
-
D-L malic acid 2.5 g (
Prolabo
20 365.233)
-
Ammonium sulphate 1.25 g (
Merck
1211)
-
Yeast extract 1 g (
BD Difco
212750)
-
Di Potassium monohydrogenophosphate 0.9 g (
VWR
26932.290)
-
Potassium di-hydrogen phosphate 0.6 g (
Labosi
P1400)
-
Magnesium sulphate heptahydrate 0.2 g (
Merck
1.05886)
-
Calcium chloride dihydrate 0.07 g (
Merck
102378)
-
E.D.T.A 0.02 g (
Sigma
E-5134)
-
Ferric citrate 0.01 g (
Merck
3862)
-
Vitamins solution - M0233 7.5 ml
-
Mineral solution - M0233 1 ml
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 6.9 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically mineral and vitamins solutions
Vitamins solution - M0233 7.5 ml
-
Thiamine monophosphate chloride 0.4 g (
Sigma
T-8637)
-
Nicotinic acid 0.2 g (
Calbiochem
481918)
-
Niacinamide (vit B3) 0.2 g (
Sigma
N-5535)
-
Biotin 8 mg (
Merck
1.24514)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Filter sterilize
Mineral solution - M0233 1 ml
-
E.D.T.A 0.3 g (
Sigma
E-5134)
-
Ferric citrate 0.05 g (
Merck
3862)
-
Calcium chloride dihydrate 0.02 g (
Merck
102378)
-
Manganese II sulphate monohydrate 2 mg (
Merck
1.05963)
-
Ammonium molybdate tetrahydrate 2 mg (
Sigma
A-7302)
-
Boric acid 1 mg (
Prolabo
20 185.297)
-
Copper II sulphate pentahydrate 1 mg (
Prolabo
23 174.290)
-
Zinc sulphate heptahydrate 1 mg (
Prolabo
29 158.237)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Autoclave at 110°C for 20 minutes
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