CRBIP-Catalogue

Formulation of medium : 691

MEDIUM691 - for Flavobacterium glaciei

- Soytone bacto 5 g (^{Becton Dickinson} 211677)
- Beef extract 3 g (^Oxoïd L29)
- Yeast extract 0.2 g (^{BD Difco} 212750)
- Glucose 5 g (^Sigma G8270)
- Sodium Chloride (P) 0.5 g (^Prolabo 27810295)
- Magnesium sulphate heptahydrate 1.5 g (^Merck 1.05886)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Distilled water 1 l

pH final : 7.0 Sterilization : Time : 15 min Temperature : 121°C OPERATING PROCEDURE 1. Boil 800 ml of distilled water in a bécher. 2. Add all products and mix until complete dissolution 3. Add distilled water until the maximum volume 4. Adjust pH 5. Divide in 17 ml tubes, 8 ml per tube 6. Sterilize 7. After sterilization, place the tube on a slightly inclined slope