Formulation of medium : 695
MEDIUM 695 - for Alkalimonas
-
Tryptone 1 g (
BECTON DICKINSON
211705)
-
Yeast extract 5 g (
BD Difco
212750)
-
Sodium chloride 10 g (
SIGMA -ALDRICH
31434-1KG-R)
-
Magnesium sulphate heptahydrate 0.2 g (
Merck
1.05886)
-
Di Potassium monohydrogenophosphate 1 g (
VWR
26932.290)
-
Bacto Agar 15 g (
BD Difco
214010)
-
Distilled water 1 l
-
Sodium carbonate hydrogen 10 g (
Merck
1.06329)
pH final : 9,0 ± 0,2 Sterilization Time : 15 min Temperature : 121°C OPERATING PROCEDURE 1. Boil 800 ml of distilled water in a bécher 2. Add all products and mix until complete dissolution 3. Add distilled water until maximum volume 4. Adjust the pH 5. Divide into 17 ml screw tubes, 8 ml per tube 6. Sterilize 7. Place the tubes on a slightly inclined slope CONTROL Make a sterility test. CONSERVATION Temperature : + 20 °C.
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