Formulation of medium : 696
MEDIUM 696 -for Bacillus quingdaonensis
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Yeast extract 10 g (
BD Difco
212750)
-
Tryptone 2.5 g (
BECTON DICKINSON
211705)
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Sodium chloride 120 g (
SIGMA -ALDRICH
31434-1KG-R)
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Magnesium sulphate heptahydrate 10 g (
Merck
1.05886)
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Potassium chloride 5 g (
Sigma
P9541)
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Calcium chloride dihydrate 0.2 g (
Merck
102378)
-
Bacto Agar 20 g (
BD Difco
214010)
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Distilled water 1 l
pH final : 7 ,4 ± 0,2 Sterilization Time : 15 min Temperature : 121°C OPERATIONAL PROCEDURE 1. Boil 800 ml of distilled water in a bécher 2. Add all products and mix until complete dissolution 3. Add distilled water until maximum volume 4. Adjust the pH 5. Divide in 17 ml screw tubes, 8 ml per tube 6. Sterilize 7. Place the tubes on a slightly inclined slope CONTROL Make a sterility test.
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