CRBIP-Catalogue

Formulation of medium : 698

MEDIUM 698 - for Rhodobacter vinaykumarii

- Potassium di-hydrogen phosphate 0.5 g (^Labosi P1400)
- Magnesium chloride hexahydrate 1 g (^Merck 1.05833)
- Sodium chloride 20 g (^{SIGMA -ALDRICH} 31434-1KG-R)
- Ammonium chloride 0.6 g (^Sigma 09718)
- Calcium chloride tetrahydrate 0.15 g (^Merck 1.02384.0100)
- Sorbitol 3 g (^Prolabo 28 210.265)
- Sodium Pyruvate 0.4 g (^Sigma P-2256)
- Ferric citrate 0.1 % solution - M00885 5 ml
- Bacto Agar 15 g (^{BD Difco} 214010)
- Distilled water 1 l
- Mineral trace elements solution - M00959 1 ml

After autoclaving, add SL7 Trace Element Solution pH final : 6,8 Sterilization Time : 15 min Temperature : 121°C OPERATING PROCEDURE 1. Pour 800 ml of distilled water in a bécher. 2. Add all products except SL7 solution and mix until complete dissolution 3. Complete with distilled water until maximum volume 4. Adjust PH 5. Pack in two 500 ml vials 6. Sterilize 7. After sterilization, place the vials in a bain-marie at 50°C 8. Once the temperature reached, add SL7 solution aseptically 9. Pack in tubes, pour 8 ml / tube CONTROLE Make a sterility test. CONDITIONS DE CONSERVATION Temperature : + 20 °C.

Ferric citrate 0.1 % solution - M00885 5 ml

- Ferric citrate 0.1 g (^Merck 3862)
- Ultra pure water make up to 100 ml (^Millipore MilliQ)

Autoclave at 121°C for 15 minutes.

Mineral trace elements solution - M00959 1 ml

- Hydrochloric acid 25% 10 g (^Prolabo 20 257.296)
- Ferrous chloride tetrahydrate 1.5 g (^Merck 1.03861)
- Cobalt II chloride hexahydrate 190 mg (^Sigma C-2644)
- ManganeseII chloride tetrahydrate 100 mg (^Prolabo 25 222.233)
- Zinc chloride 70 mg (^Merck 1.08816)
- Boric acid 62 mg (^Prolabo 20 185.297)
- Sodium molybdate dihydrate 36 mg (^Merck 6521)
- Nickel II chloride hexahydrate 24 mg (^Prolabo 25 850.290)
- Copper II chloride dihydrate 17 mg (^Merck 2733)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Adjust pH to 7.2 + 0.1