CRBIP-Catalogue

Formulation of medium : 7

MEDIUM 7 - for Actinomyces

- Heart infusion broth 25 g (^{Becton Dickinson} 238400)
- Yeast extract 5 g (^{BD Difco} 212750)
- Casitone 4 g (^{Becton Dickinson} 225930)
- L-Cystein 1 g (^Sigma C7352)
- Glucose 5 g (^Sigma G8270)
- Starch maize 1 g (^Prolabo 21 145.296)
- Di Potassium monohydrogenophosphate 15 g (^VWR 26932.290)
- Ammonium sulphate 1 g (^Merck 1211)
- Magnesium sulphate heptahydrate 0.2 g (^Merck 1.05886)
- Calcium chloride dihydrate 0.02 g (^Merck 102378)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Ultra pure water make up to 1000 ml (^Millipore MilliQ)

Dissolve agar at boiling Cool to 50°C Add all products Adjust pH to 7.2 + 0.2 Autoclave at 121°C for 15 minutes