Formulation of medium : 70
MEDIUM 70 - columbia agar with horse blood and hemin for Bartonella
-
Columbia agar 39 g (
Bio-Rad
279240)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
-
Horse blood 100 ml (
BioMérieux
55 833)
-
Hemin solution - M00149 10 ml
Add all products except horse blood and hemin solution Adjust pH to 7.3 + 0.2 Autoclave at 121°C for 15 minutes Cool to 50°C Add aseptically horse blood and hemin solution
Hemin solution - M00149 10 ml
-
Hemin chloride 0.1 g (
Merck
3741)
-
Sodium hydroxyde 30% 0.1 ml (
Fisher
S/4950/PB15)
-
Triethanolamine 4 ml (
VWR
28743.290)
-
Ultra pure water make up to 96 ml (
Millipore
MilliQ)
Filter sterilize
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