CRBIP-Catalogue

Formulation of medium : 701

MEDIUM 701 - for -Limnicola

- Magnesium sulphate heptahydrate 75 mg (^Merck 1.05886)
- Calcium nitrate tetrahydrate 43 mg (^Prolabo 22 384.298)
- Sodium carbonate decahydrate 16 mg (^Merck 1.06391.1000)
- Potassium chloride 5 mg (^Sigma P9541)
- Potassium di-hydrogen phosphate 3.7 mg (^Labosi P1400)
- E.D.T.A tetrasodique 4.4 mg (^Sigma E6511)
- Ferric III chloride hexahydrate 3.2 mg (^Prolabo 24 208.237)
- Boric acid 1 mg (^Prolabo 20 185.297)
- ManganeseII chloride tetrahydrate 0.2 mg (^Prolabo 25 222.233)
- Nickel II chloride hexahydrate 0.1 mg (^Prolabo 25 850.290)
- Zinc sulphate heptahydrate 0.02 mg (^Prolabo 29 158.237)
- Copper II sulphate pentahydrate 0.02 mg (^Prolabo 23 174.290)
- Cobalt II chloride anhydrous 0.01 mg (^Merck 802540)
- Sodium molybdate dihydrate 0.006 mg (^Merck 6521)
- Brain heart infusion 1 g (^{BECTON DICKINSON} 237500)
- Soytone peptone 1 g (^{BECTON DICKINSON} 243620)
- Yeast extract 1 g (^{BD Difco} 212750)
- Bacto Agar 15 g (^{BD Difco} 214010)
- Distilled water 1 l

pH final : 7,2 + 0,2 Sterilization Time : 15 min Temperature : 121°C OPERATIONAL PROCEDURE 1. Boil 800 ml of distilled water in a bécher 2. Add all products and mix until complete dissolution 3. Add distilled water until maximum volume 4. Adjust the pH 5. Divide into 17 ml screw tubes, 8 ml per tube 6. Sterilize 7. Place the tubes on a slightly inclined slope CONSERVATION Temperature : + 20 °C.