Formulation of medium : 719
MEDIUM 719 - for - Propioniferax
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Trypto casein soy broth 30 g (
Bio-Rad
64144)
-
Yeast extract 3 g (
BD Difco
212750)
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Bacto Agar 15 g (
BD Difco
214010)
-
Ultra pure water make up to 1 l (
Millipore
MilliQ)
pH final : 7,0 0,2 Sterilization Temperature : + 20°C Time : … min OPERATING PROCEDURE 1. Boil 800 ml of distilled water in a bécher 2. Add all the products and mix until complete dissolution 3. Complete with distilled water until the maximum volume 4. Adjust pH 5. Split in 17 ml screw tubes, 8 ml per tubes 6. Sterilize 7. Place the tubes on a slighty inclined slope CONDITIONS OF CONSERVATION Temperature : + 20°C.
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