Formulation of medium : 79
MEDIUM 79 - for Mycoplasma
-
PPLO broth 0.5 g (
Difco
255420)
-
Soytone bacto 5.3 g (
Becton Dickinson
211677)
-
Tryptone 10 g (
BECTON DICKINSON
211705)
-
Glucose 5 g (
Sigma
G8270)
-
Ultra pure water make up to 610 ml (
Millipore
MilliQ)
-
Sterile supplement - M00265 375 ml
Add all products except Sterile supplement Adjust pH to 7.8 + 0.2 Autoclave at 121°C for 15 minutes Cool to 45°C Add aseptically Sterile supplement
Sterile supplement - M00265 375 ml
-
CMRL-1066 (10X) without L-glutamine 50 ml (
Gibco
21540-026)
-
Yeast extract solution 25 % - M00263 35 ml
-
TC Yeastolate 2 % solution - M00266 100 ml
-
Calf serum 170 ml (
Gibco
10099-141)
-
0.1 % Phenol red - M00387 20 ml
-
Ampicillin 1 g (
Bristol-myers squibb
4 3244576 000197 00)
Filter sterilize
Yeast extract solution 25 % - M00263 35 ml
-
Yeast extract 250 g (
BD Difco
212750)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
TC Yeastolate 2 % solution - M00266 100 ml
-
Yeastolate 10 g (
Becton Dickinson
255772)
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
0.1 % Phenol red - M00387 20 ml
-
Sodium hydroxyde 5 ml (
Prolabo
28 252.293)
-
Phenol red 500 mg (
Merck
7241)
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
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