Formulation of medium : 80
MEDIUM 80 - for Ureaplasma urealyticum
-
Trypto casein soy broth 24 g (
Bio-Rad
64144)
-
Yeast extract solution 25 % - M00263 20 ml
-
0.1 % Phenol red - M00387 20 ml
-
Ultra pure water make up to 740 ml (
Millipore
MilliQ)
-
Sterile supplement - M00270 220 ml
Add all products except sterile supplement Adjust pH to 6.0 + 0.2 Autoclave at 121°C for 15 minutes Cool to 45°C Add aseptically sterile supplement
Yeast extract solution 25 % - M00263 20 ml
-
Yeast extract 250 g (
BD Difco
212750)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
0.1 % Phenol red - M00387 20 ml
-
Sodium hydroxyde 5 ml (
Prolabo
28 252.293)
-
Phenol red 500 mg (
Merck
7241)
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
Sterile supplement - M00270 220 ml
-
Horse serum 200 ml (
Gibco
26050-088)
-
Urea solution 20% - M0090 10 ml
-
Cysteine hydrochloride 5 % solution - M0172 10 ml
-
Ampicillin 1 g (
Bristol-myers squibb
4 3244576 000197 00)
Filter sterilze
Urea solution 20% - M0090 10 ml
-
Urea 20 g (
Carlo Erba
490758)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Filter sterilize
Cysteine hydrochloride 5 % solution - M0172 10 ml
-
L-Cystein 10 g (
Sigma
C7352)
-
Ultra pure water make up to 200 ml (
Millipore
MilliQ)
Filter sterilize
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