Formulation of medium : 89
MEDIUM 89 - for Mycoplasma
-
PPLO broth 17 g (
Difco
255420)
-
Yeast extract solution 25 % - M00263 100 ml
-
0.1 % Phenol red - M00387 20 ml
-
Ultra pure water make up to 670 ml (
Millipore
MilliQ)
-
Sterile supplement - M00276 210 ml
Add all components except sterile supplement Adjust pH to 7.2 + 0.2 Autoclave at 121°C for 15 minutes Add aseptically sterile supplement
Yeast extract solution 25 % - M00263 100 ml
-
Yeast extract 250 g (
BD Difco
212750)
-
Ultra pure water make up to 1000 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
0.1 % Phenol red - M00387 20 ml
-
Sodium hydroxyde 5 ml (
Prolabo
28 252.293)
-
Phenol red 500 mg (
Merck
7241)
-
Ultra pure water make up to 500 ml (
Millipore
MilliQ)
Adjust pH to 7.0 + 0.2 Autoclave at 121°C for 15 minutes.
Sterile supplement - M00276 210 ml
-
Horse blood 200 ml (
BioMérieux
55 833)
-
Arginine 50 % solution - M00277 10 ml
-
Ampicillin 1 g (
Bristol-myers squibb
4 3244576 000197 00)
Arginine 50 % solution - M00277 10 ml
-
L-Arginine hydrochloride 50 g (
Gibco
21009.014)
-
Ultra pure water make up to 100 ml (
Millipore
MilliQ)
Autoclave at 121°C for 15 minutes.
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